Michaelis-Menten vs. Lineweaver-Burk Plot: Fast Way to Find Km & Vmax
The Michaelis-Menten plot shows reaction velocity (V) vs substrate concentration ([S]) and fits data to V = Vmax[S]/(Km + [S]). The Lineweaver-Burk plot flips the same equation into a straight line: 1/V vs 1/[S] with slope = Km/Vmax and y-intercept = 1/Vmax. Bench scientists often grab whichever plot their mentor used first; a sloppy axis…