Agarose vs. Polyacrylamide Gel Electrophoresis: Key Differences & Best Uses
Agarose gel electrophoresis uses a seaweed-derived sugar matrix to separate large DNA or RNA fragments by size under an electric field. Polyacrylamide gel electrophoresis employs synthetic cross-linked acrylamide to resolve much smaller nucleic acids or proteins with higher resolution. Lab newbies often grab whichever gel is on the shelf, but picking the wrong one can…